Identification of the copper chaperone, CUC‐1, in Caenorhabditis elegans: tissue specific co‐expression with the copper transporting ATPase, CUA‐1
Open Access
- 27 November 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 440 (1-2) , 141-146
- https://doi.org/10.1016/s0014-5793(98)01431-8
Abstract
A cDNA encoding a putative copper chaperone protein, CUC‐1, was cloned from Caenorhabditis elegans. CUC‐1 had the characteristic motifs of MTCXXC and KKTGK, and showed 49.3 and 39.1% sequence identity with yeast Atx1p and human HAH1, respectively. Expression of CUC‐1 cDNA complemented a null atx1 mutant, the yeast copper chaperone gene, thus demonstrating that CUC‐1 is a functional copper chaperone. Studies with transgenic worms indicated that cuc‐1 and cua‐1, which encodes the copper transporting ATPase, are expressed together in intestinal cells of adult and hypodermal cells in the larvae. cua‐1 was also expressed in pharyngeal muscle but cuc‐1 was not. These results suggest that CUC‐1 and CUA‐1 constitute a copper trafficking pathway similar to the yeast counterparts in intestinal and hypodermal cells, and CUA‐1 may have a different function in pharyngeal muscle.Keywords
This publication has 27 references indexed in Scilit:
- Functional Expression of the Menkes Disease Protein Reveals Common Biochemical Mechanisms Among the Copper-transporting P-type ATPasesJournal of Biological Chemistry, 1998
- Essential Cys-Pro-Cys Motif ofCaenorhabditis elegansCopper Transport ATPaseBioscience, Biotechnology, and Biochemistry, 1998
- Identification and Functional Expression of HAH1, a Novel Human Gene Involved in Copper HomeostasisJournal of Biological Chemistry, 1997
- SCO1 and SCO2 Act as High Copy Suppressors of a Mitochondrial Copper Recruitment Defect in Saccharomyces cerevisiaeJournal of Biological Chemistry, 1996
- A Permease-Oxidase Complex Involved in High-Affinity Iron Uptake in YeastScience, 1996
- 2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegansNature, 1994
- Isolation and Characterization of a Human Liver cDNA as a Candidate Gene for Wilson DiseaseBiochemical and Biophysical Research Communications, 1993
- Isolation of a candidate gene for Menkes disease and evidence that it encodes a copper–transporting ATPaseNature Genetics, 1993
- Isolation of a partial candidate gene for Menkes disease by positional cloningNature Genetics, 1993
- Isolation of a candidate gene for Menkes disease that encodes a potential heavy metal binding proteinNature Genetics, 1993