Identification of Legume Nodule Bacteroids by the Fluorescent Antibody Reaction

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Abstract
Summary: A fluorescent antibody technique which permits rapid identification of legume nodule bacteroids and which permits the separation of bacteroid material and laboratory grown cultures of close serological affinity is described. Specificity of fluorescein isothiocyanate γ‐globulin conjugates was increased with dilution. The ability of a medium sized clover nodule to produce sufficient bacteroid material for 20 fluorescent antibody stains allows increased accuracy and scope of the method. A single operator in 1 day may test > 400 nodules for one or more symbionts.