Enzymatic synthesis of RNA oligonucleotides
- 1 January 1987
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (16) , 6705-6711
- https://doi.org/10.1093/nar/15.16.6705
Abstract
Using SP6 RNA polymerase and various synthetic DNA oligomers as templates (12-65 b), we have obtained efficient synthesis of RNA transcripts without the need for either primer or promoter. Gel analysis of transcripts made from templates of known size demonstrated a predominant single species, together with some minor bands. For a given template the major band had the same 5'-nucleotide as predicted from the 3'-nucleotide of the template. This synthesis procedure makes it possible to efficiently and conveniently make labeled or unlabeled RNA from synthetic DNA oligonucleotides.Keywords
This publication has 10 references indexed in Scilit:
- [57] Sequencing end-labeled DNA with base-specific chemical cleavagesPublished by Elsevier ,2004
- Self-cleavage of plus and minus RNA transcripts of avocado sunblotch viroidNucleic Acids Research, 1986
- A novel transcription property of SP6 and T7 RNA polymerases: dependence on template structureNucleic Acids Research, 1985
- Contributions of dangling end stacking and terminal base-pair formation to the stabilities of XGGCCp, XCCGGp, XGGCCYp, and XCCGGYp helixesBiochemistry, 1985
- Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoterNucleic Acids Research, 1984
- The effects of base sequence and dangling bases on the stability of short ribonucleic acid duplexes.1980
- Phy M: an RNase activity specific for U and A residues useful in RNA sequence analysisNucleic Acids Research, 1980
- [38] Fractionation of low molecular weight DNA or RNA in polyacrylamide gels containing 98% formamide or 7 M ureaPublished by Elsevier ,1980
- Termination of transcription by Escherichia coli RNA polymerase in vitro is affected by ribonucleoside triphosphate base analogs.Journal of Biological Chemistry, 1978
- Mechanism of RNA polymerase action: Formation of DNA-RNA hybrids with single-stranded templatesJournal of Molecular Biology, 1964