Quantitative studies of integration of murine leukemia virus after exogenous infection.

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Abstract
Using a [3H]DNA probe prepared from AKR murine leukemia virus, the number of copies of the AKR virus genome integrated into the cellular DNA after exogenous infection of NIH and AKR mouse embryo, and Fisher rat embryo cells in tissue culture was determined. NIH mouse cells, which lack a portion of the viral genome (referred to as Gross-AKR specific sequences), incorporated 3-4 copies of these sequences/haploid genome. AKR cells, in which the Gross-AKR specific sequences are already present as 3-4 copies/haploid genome, did not show any distinct change in copy number after infection. Rat cells, which lack DNA sequences homologous to murine leukemia virus, incorporated 1 copy of the viral genome/haploid genome. The presence of viral sequences may affect the efficiency of integration of exogenous provirus and there may be a limit to the number of copies that can be inserted.