Ethylcarbamoyl protection for cysteine in the preparation of peptide‐conjugate immunogens
- 1 July 1992
- journal article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 40 (1) , 62-65
- https://doi.org/10.1111/j.1399-3011.1992.tb00105.x
Abstract
During the solid‐phase synthesis of over 100 peptides, we have observed that the ethylcarbamoyl group is useful for the side chain protection of cysteine in peptides containing a single cysteine residue. The ethylcarbamoyl group is stable to the conditions of acidolytic cleavage, purification and long term storage. Brief treatment of peptides containing an S‐ethylcarbamoylcysteine residue with aqueous sodium hydroxide gives the deprotected cysteine peptide that can be coupled to carrier molecules such as proteins to give immunogen conjugates.Keywords
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