Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells
Top Cited Papers
Open Access
- 27 December 2005
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 172 (1) , 91-102
- https://doi.org/10.1083/jcb.200508044
Abstract
The growth and repair of skeletal muscle after birth depends on satellite cells that are characterized by the expression of Pax7. We show that Pax3, the paralogue of Pax7, is also present in both quiescent and activated satellite cells in many skeletal muscles. Dominant-negative forms of both Pax3 and -7 repress MyoD, but do not interfere with the expression of the other myogenic determination factor, Myf5, which, together with Pax3/7, regulates the myogenic differentiation of these cells. In Pax7 mutants, satellite cells are progressively lost in both Pax3-expressing and -nonexpressing muscles. We show that this is caused by satellite cell death, with effects on the cell cycle. Manipulation of the dominant-negative forms of these factors in satellite cell cultures demonstrates that Pax3 cannot replace the antiapoptotic function of Pax7. These findings underline the importance of cell survival in controlling the stem cell populations of adult tissues and demonstrate a role for upstream factors in this context.Keywords
This publication has 59 references indexed in Scilit:
- Direct Isolation of Satellite Cells for Skeletal Muscle RegenerationScience, 2005
- Pax3/Pax7 mark a novel population of primitive myogenic cells during developmentGenes & Development, 2005
- Coordination of cell death and the cell cycle: linking proliferation to death through private and communal couplersCurrent Opinion in Cell Biology, 2004
- Paired-Box genes are frequently expressed in cancer and often required for cancer cell survivalOncogene, 2003
- MyoD induces apoptosis in the absence of RB function through a p21WAF1-dependent re-localization of cyclin/cdk complexes to the nucleusOncogene, 2002
- The Ki-67 protein: From the known and the unknownJournal of Cellular Physiology, 2000
- Primary renal hypoplasia in humans and mice with PAX2 mutations: evidence of increased apoptosis in fetal kidneys of Pax21Neu +/- mutant miceHuman Molecular Genetics, 2000
- MyoD, Myogenin, and Desmin-nls-lacZ Transgene Emphasize the Distinct Patterns of Satellite Cell Activation in Growth and RegenerationExperimental Cell Research, 1998
- Construction of adenoviral and retroviral vectors coexpressing the genes encoding the hepatitis B surface antigen and B7-1 proteinGene, 1996
- Pax: Genes for mice and menPharmacology & Therapeutics, 1994