SOLUBILIZATION OF CELL ANTIGENS ASSOCIATED WITH THE REJECTION OF TRANSFERRED RABBIT LYMPH NODE CELLS

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Abstract
In a study of solubilization of the cell antigens associated with the rejection of transferred rabbit lymph node cells, fragments of such cells, obtained by centrifugation of cell-wash fluids, were extracted with Triton, and the water-in-soluble part of the Triton extract was solubilized with snake venom. Two fractions were obtained by this procedure, within the same range of yield: a saline-soluble fraction of the Triton extract, and a venom-solubilized fraction. Both of these were tested for serologic activity against rabbit anti-rabbit-leucocyte serum, which can cause suppression of transferred lymph node cells, and against suppressive antibody specifically purified from such sera. Both preparations were found to give positive results in the following tests: agglutination of RBC coated with the suppressive antibody, adsorption to RBC with resulting agglutinability of the cells by the anti-leucocyte sera, and inhibition of such adsorption-hemagglutination by solutions of the antigens. Experiments were also carried out on the association of these antigens with the rejection of transferred lymph node cells. Both preparations were found to absorb from rabbit anti-rabbit-leucocyte serum the antibody which causes suppression of transferred lymph node cells. Also, sera of rabbits given secondary injections of these preparations, after primary injections of rabbit leucocytes, were found to suppress transferred lymph node cells.

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