A method for quantitation of vitamin B2 by using an enzyme (producing vitamin B2-aldehyde) from Schizophyllum commune.
- 1 January 1980
- journal article
- research article
- Published by Center for Academic Publications Japan in Journal of Nutritional Science and Vitaminology
- Vol. 26 (5) , 419-426
- https://doi.org/10.3177/jnsv.26.419
Abstract
A method for enzymatic determination of riboflavin was developed by enzymatic reduction of 2,6-dichlorophenolindophenol using a vitamin B2-aldehyde-forming enzyme prepared from S. commune. The enzymatic method enabled the determination of riboflavin in a mixture containing riboflavin, FMN and FAD without separation of riboflavin. The total amount of flavins in the mixture was determined by the enzyme in the presence of phosphatase.This publication has 3 references indexed in Scilit:
- Electrophoretic separation and determination of flavinsBiochemical Journal, 1955
- Studies on Thiols. I. Oxidation of Thiol Groups by 2,6-Dichlorophenol Indophenol1Journal of the American Chemical Society, 1955
- THE FLUOROMETRIC MEASUREMENT OF THE NUCLEOTIDES OF RIBOFLAVIN AND THEIR CONCENTRATION IN TISSUESJournal of Biological Chemistry, 1949