A new HLA–DQB 1*0306 allele sharing motifs from DQB 1*03032 and DQB1*04 sequences
- 1 November 1996
- journal article
- Published by Wiley in Tissue Antigens
- Vol. 48 (5) , 580-585
- https://doi.org/10.1111/j.1399-0039.1996.tb02672.x
Abstract
We have discovered a new HLA‐DQB 1 allele in a Japanese family, MAT. In the family the new allele segregates in three generations and demonstrates the positive association with DRB 1*0901. We observed a novel RFLP pattern in the course of examining the modified PCR‐RFLP method for HLA‐DQB 1 genotyping. The PCR‐SSOP analysis also showed a new hybridized pattern. Sequence analysis of the allele indicates that it was generated by a gene conversion‐like event between the HLADQB 1*03032 and one of DQB 1*04 contemporary alleles. This new allelic product did not react with all of allosera and monoclonal antibodies against DQ1, DQ2, DQ3, DQ4 and DQ7. The HLA molecule encoded by the allele is not defined by serology. This new allele was officially recognized and named DQB 1*0306 by the WHO Nomenclature Committee in November 1995.Keywords
This publication has 23 references indexed in Scilit:
- HLA Class II region nucleotide sequences, 1995Tissue Antigens, 1995
- HLA‐DQB1 and ‐DQA1 typing by PCR amplification with sequence‐specific primers (PCR‐SSP) in 2 hoursTissue Antigens, 1993
- Thermal neutron capture therapy of malignant melanoma using 10B-monoclonal antibodiesMelanoma Research, 1992
- Nomenclature for factors of the HLA system, 1990Human Immunology, 1991
- HLA-DR generic typing by AFLPTissue Antigens, 1991
- HLA-DP typing by amplified fragment length polymorphisms (AFLPs)Immunogenetics, 1990
- A simple and rapid method for HLA‐DRB and ‐DQB typing by digestion of PCR‐amplified DNA with allele specific restriction endonucleasesTissue Antigens, 1990
- HLA-DR and -DQ epitopes and monoclonal antibody specificityImmunology Today, 1989
- HUMAN CLASS II MAJOR HISTOCOMPATIBILITY COMPLEX GENES AND PROTEINSAnnual Review of Biochemistry, 1988
- Mapping of the class II region of the human major histocompatibility complex by pulsed-field gel electrophoresisNature, 1986