Extensive homologous chloroplast DNA recombination in the pt14 Nicotiana somatic hybrid
- 1 January 1990
- journal article
- research article
- Published by Springer Nature in Theoretical and Applied Genetics
- Vol. 79 (1) , 28-32
- https://doi.org/10.1007/bf00223782
Abstract
In a previous study, six recombination sites have been confirmed in the chloroplast DNA (cpDNA) of pt14, a somatic hybrid of Nicotiana tabacum and Nicotiana plumbaginifolia. In the present study, physical mapping revealed six recombination sites in the 11.4-kb SalI fragment alone, only one of which has been previously identified. This fragment is located in the large unique region. We assume, therefore, that the pt14 cpDNA is a fine mosaic of the parental genomes with a recombination site about every 2 kb. A 748-bp region that comprised the intergenic region between ORF73 and ORF74B, and 460 bp of the petD intron have been sequenced. Parent-specific sequences in the pt14 DNA defined the regions within which recombination took place. The exact site of recombination events could not be determined because the parental sequences were identical between the polymorphic markers, and these sequences have been preserved in the pt14 line.This publication has 22 references indexed in Scilit:
- Limited chloroplast gene transfer via recombination overcomes plastomegenome incompatibility between Nicotiana tabacum and Solanum tuberosumPlant Molecular Biology, 1989
- Unidirectional gene conversions in the chloroplast of Chlamydomonas interspecific hybridsMolecular Genetics and Genomics, 1988
- A point mutation in the chloroplast 16 S rRNA gene of a streptomycin resistant Nicotiana tabacumFEBS Letters, 1987
- COMPARATIVE ORGANIZATION OF CHLOROPLAST GENOMESAnnual Review of Genetics, 1985
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Deleted forms of plastid DNA in albino plants from cereal anther cultureCurrent Genetics, 1985
- Chloroplast DNA deletions associated with wheat plants regenerated from pollen: possible basis for maternal inheritance of chloroplastsCell, 1984
- Physical mapping of plastid DNA variation among eleven Nicotiana speciesTheoretical and Applied Genetics, 1984
- Construction of the physical map of the chloroplast DNA of Phaseolus vulgaris and localization of ribosomal and transfer RNA genesGene, 1983
- Chloroplast DNA exists in two orientationsNature, 1983