Immunogold detection of two neurohormones: the locust ovary maturing parsin and neuroparsin

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Abstract
The cellular localization of two neurohormones of the locust pars intercerebralis-corpora cardiaca system: the ovary maturing parsin and neuroparsin, was investigated using electron microscopic immunocytochemistry (post-embedding immunogold labelling). The ovary maturing parsin and neuroparsin containing cells were first identified in semithin sections treated by combined histochemical- and immunostaining. The neuroparsin cells were paraldehyde fuchsin positive (A-type cells) and the ovary maturing parsin cells were paraldehyde fuchsin negative when semithin sections were stained with paraldehyde fuchsin and immunostained with anti-ovary maturing parsin serum. The ovary maturing parsin and neuroparsin producing cells were identified on immunogold labelled ultrathin sections adjacent to double stained semithin sections. Ovary maturing parsin cells have larger more numerous vesicles of greater electron density than neuroparsin cells. The neuroparsin cells contained more lysosomal structures than the ovary maturing parsin cells suggesting different neurosecretory dynamics. Thus, immunogold labelling with antisera specific for each neurohormone demonstrates the individual nature of these two neurosecretory cells in the pars intercerebralis of the Locust.