Effect of Chlorambucil on Thymidine Utilization by Yoshida Ascites Tumor Cells 2

Abstract
The effects of chlorambucil treatment on thymidine utilization by 2 strains (one sensitive and the other refractory) of the Yoshida ascites sarcoma in rats were described. In drug-sensitive cells, chlorambucil treatment caused elevated deoxyribonucleoside triphosphate pools and increased activity of thymidylate-synthesizing enzymes coincided with an enhanced rate of DNA synthesis. Resistant cells maintained normal deoxyribonucleoside triphosphate pools after chlorambucil treatment when the activity of enzymes synthesizing thymidylate was decreased. A possible explanation of this latter observation was the elevated DNAse II activity in resistant cells after drug treatment.

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