Aphanomycesroot rot of peas 1 . Evaluation of methods for assessing inoculum density ofAphanomyces euteichesin soil
Open Access
- 1 May 1987
- journal article
- research article
- Published by Taylor & Francis in New Zealand Journal of Agricultural Research
- Vol. 30 (2) , 213-217
- https://doi.org/10.1080/00288233.1987.10430499
Abstract
Soil samples from 18 Aphanomyces infested fields were tested by the disease severity index (DSI) method and by direct counting of oospores. There was a curvilinear relationship (r = 0.97***) between DSI and the number of oospores in soil. This was confirmed when a known number of oospores produced in culture were added to non-infested soil. Both assays were suitable techniques for quantifying the inoculum density of Aphanomyces euteiches in soil. The methods could also accurately assess the changes in a population of the pathogen when subjected to experimental soil treatments especially in soil with low DSI values. The Most Probable Number method of estimating inoculum density was less satisfactory.Keywords
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