INVITRO PRODUCTION OF IGE BY HUMAN PERIPHERAL-BLOOD MONONUCLEAR-CELLS .2. CELLS INVOLVED IN THE SPONTANEOUS IGE PRODUCTION IN ATOPIC PATIENTS

  • 1 January 1980
    • journal article
    • research article
    • Vol. 42  (3) , 579-588
Abstract
Spontaneous IgE production in vitro was investigated in 7 day cultures of unfractionated mononuclear cells (MNC) and MNC subpopulations from atopic patients. Depletion of either phagocytic or adherent cells decreased the IgE amount detectable in 7 day culture supernatants, but this decrease was due, at least in part, to a loss of cytophilic IgE. Ig bearing cell (SIg+) depletion reduced significantly but did not abolish the spontaneous IgE production in vitro. On the other hand, depletion of IgM-bearing lymphocytes (SIgM+), which virtually abolished the production of IgM class Ig, did not significantly change spontaneous IgE production. Similarly, no change in spontaneous IgE production was found when lymphocyte suspensions were depleted of complement receptor-bearing cells (CR+). In contrast, spontaneous IgE production was significantly increased by T lymphocyte depletion and this increase did not reflect the enrichment for IgE-producing cells caused by the fractionation procedure. No change in spontaneous IgE production was found when small numbers of autologous T lymphocytes were added to B cell fractions, whereas the addition of higher concentrations of autologous T cells induced a marked spontaneous IgE production inhibition. The addition in culture of pokeweed mitogen (PWM) resulted in a marked reduction of the spontaneous IgE production by B cells, also in the presence of small concentrations of autologous T lymphocytes. Normal T cells were consistently effective in inducing a partial inhibition of the spontaneous IgE production by B cells from atopic patients, whereas T cells from a noticeable proportion of atopic patients were not. Apparently MNC responsible for spontaneous IgE production in atopic subjects are SIgM- and CR-deficient well-differentiated lymphocytes which probably represent the result of an in vivo activation. This spontaneous IgE production can still be influenced by in vitro manipulation, such as variations in T-B cell ratios or addition of PWM. Apparently normal T cells are generally more effective than T cells from atopic patients in regulating the activity of spontaneous IgE-producing cells present in the blood of atopic subjects.