Chromic-Acid Formaldehyde Fixation of Nucleic Acids of Bacteriophage 6 and Infectious Bovine Rhinotracheitis Virus
- 1 May 1978
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 39 (2) , 377-380
- https://doi.org/10.1099/0022-1317-39-2-377
Abstract
Bacteriophage phi6 nucleic acid was present as a torus after chromic acid-formaldehyde-OSO4 fixation and acetone and propylene oxide dehydration. A herpes virus, infectious bovine rhinotracheitis virus, had its DNA mostly as a torus, collapsed in the centre, or as a network, after glutaraldehyde-OSO4 fixation, but in an uncollapsed torus or network formation after chromic acid-formaldehyde-OSO4. This fixative stabilized nucleic acids, allowing acetone dehydration and plastic embedding without collapse of nucleic acid to the centre of the virion.Keywords
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