Simple phagemid-based system for generating allele replacements in Escherichia coli
Open Access
- 1 July 1993
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 175 (13) , 4260-4262
- https://doi.org/10.1128/jb.175.13.4260-4262.1993
Abstract
We describe a phagemid-based system for rapidly generating chromosomal allele replacements in two steps. The system utilizes simple vector-determined selection procedures for each step and requires only that the target strain be male and sup+ (nonsuppressing for amber mutations).Keywords
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