Inoculation of Chick Embryos with Sporozoites of Plasmodium gallinaceum by Inducing Mosquitoes to Feed through Shell Membrane

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Abstract
Sporozoites were transmitted to 10- to 13-day-old chick embryos by inducing infected mosquitoes (Aedes aegypti) to feed through the shell membranes. A small slit was made in the egg shell over one of the superficial chorio-allantoic blood vessels, by a dental carbon disc, care being taken not to penetrate the shell membrane. Infected mosquitoes caged in small glass lantern globes or plastic cylinders, the ends of which were covered with bobbinet, were placed over the exposed shell membrane, which had previously been moistened with saliva. A number of such mosquitoes engorged on blood from the chorio-allantoic vessels lying beneath the membranes; others fed on allan-toic fluid. Chicks hatching out from such eggs either had P. gallinaceum in their blood at the time of hatching or developed parasitemia within a few days afterwards, in a sufficient number of instances to make the procedure a practical one for inoculating chick embryos with the sporo-zoites of this species of avian malaria.
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