Novel Ligand-Exchange Chromatographic Resolution of DL-Amino Acids using Nucleotides and Coenzymes

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Abstract
A mobile phase containing either ADP, NAD, or FAD as a chiral additive, and a chiral stationary phase coated with cyanocobalamin, are adopted for ligand-exchange chromatography. Fourteen of sixteen DL-amino acids tested are resolved. Using the mobile phase, separation factor (α) and resolution rate (Rs) are known to be dependent upon the concentration of chiral additives. The chiral stationary sorbent prepared by letting cyanocobalamin flow through an ODS silica gel column could repeatedly be utilized for resolutions without further addition of any chiral additive. The results obtained are compared with those of leading ligand-exchange chromatographic studies using amino acids or their derivatives.