Minimal Biotransformation and Toxicity of Desflurane in Guinea Pig Liver Slices

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Abstract
Biotransformation and hepatotoxicity of desflurane were evaluated in the guinea pig liver slice culture system. Liver slices (250–300 μm) were prepared from 600—650-g male Hartley guinea pigs. The slices were incubated in sealed vials in a Krebs-Henseleit buffer at 37oC under 95% O2. Desflurane was vaporized to produce media concentrations of 0.7—2.3 mM. After incubation (3–24 h) viability of the slices was determined (K+ content; protein synthesis/ secretion) along with the biotransformation of desflurane (F−). Isoflurane (2.3 mM) was included in the studies for comparative purposes. Although desflurane caused a mild concentration-related reduction in slice K+ content (1.1–2.2 mM; 20%—40% of control), the effects were less than those produced by 2.3 mM isoflurane (50% of control). High concentrations of desflurane decreased protein synthesis at the first 9 h of incubation, and isoflurane decreased protein synthesis throughout the incubation period. Neither anesthetic affected protein secretion. The biotransformation of desflurane was minimal with threefold less F− produced from desflurane than isoflurane.

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