Attractants and repellents influence methylation and demethylation of methyl-accepting chemotaxis proteins in an extract of Escherichia coli.
- 1 December 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (12) , 6309-6313
- https://doi.org/10.1073/pnas.76.12.6309
Abstract
During bacterial chemotaxis, attractants and repellents alter the methylation levels of the methyl-accepting chemotaxis proteins (MCP). These methylation levels represent balance between 2 enzymatic processes: methylation and demethylation. In vivo experiments previously showed that chemoeffectors influence the demethylation process; effects on the methylation system were not reported. In a cell-free extract of E. coli both methylation and demethylation of the MCP are affected by attractants and repellents. Attractants enhance methylation and inhibit demethylation. Repellents inhibit methylation and stimulate demethylation. The cell-free system provides an opportunity for further study of the mechanisms by which attractants and repellents influence the levels of methylation of the MCP.This publication has 22 references indexed in Scilit:
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