Some properties of vacuoles isolated from Neurospora crassa slime variant
- 1 January 1979
- journal article
- research article
- Published by Springer Nature in Archiv für Mikrobiologie
- Vol. 120 (1) , 31-34
- https://doi.org/10.1007/bf00413268
Abstract
A method for the isolation of vacuoles based on polybase induced lysis of protoplasts of the cell wall deficient Neurospora crassa slime variant is described. Isolated vacuoles are characterized by 12 to 50 times increased specific activities of several hydrolases as compared with the total homogenate of protoplasts. Total α-amino nitrogen, arginine, and polyphosphate are also greatly enriched in these vacuoles. Vacuoles are equipped with a permease for the transport of basic amino acids across the tonoplast.Keywords
This publication has 19 references indexed in Scilit:
- Proteolytic Enzymes of Neurospora crassaEuropean Journal of Biochemistry, 1975
- Characterization of a Specific Transport System for Arginine in Isolated Yeast VacuolesEuropean Journal of Biochemistry, 1975
- Polybase induced lysis of yeast spheroplastsArchiv für Mikrobiologie, 1975
- Chapter 7 Isolation of Vacuoles from YeastsPublished by Elsevier ,1975
- Activities and cellular localization of yeast proteases and their inhibitorsBiochemical and Biophysical Research Communications, 1974
- Compartmentation of inhibitors of proteinases A and B and carboxypeptidase Y in yeastBiochemical and Biophysical Research Communications, 1974
- A rapid, sensitive, and specific method for the determination of protein in dilute solutionAnalytical Biochemistry, 1973
- Distribution of Wall-bound Invertase during the Asexual Life-cycle of Neurospora crassaJournal of General Microbiology, 1972
- A particulate fraction from Neurospora crassa exhibiting aryl sulfatase activityArchives of Biochemistry and Biophysics, 1971
- Intrazelluläre Lokalisatton proteolytischer Enzyme von Neurospora crassaCell and tissue research, 1965