An Electrophoretic Analysis of Superoxide Dismutase in Campylobacter spp.

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Abstract
Superoxide dismutase (SOD, superoxide:superoxide reductase, EC 1.15.1.1) activity was studied in 23 strains of Campylobacter spp. using disc polyacrylamide gel electrophoresis. Different enzyme patterns were observed with extracts of different Campylobacter spp.: 3 migration bands were found in all strains of C. sputorum ssp. sputorum and C. sputorum ssp. bubulus (relative mobilities, Rm, 0.57, 0.76 and 0.85), and C. fetus ssp. fetus (Rm 0.60, 0.72 and 0.81), while 4 migration bands (Rm 0.52, 0.57, 0.73 and 0.82) were found in C. fetus ssp. venerealis. One band (Rm 0.73) was found in C. coli CIP 7080 and 2 bands (Rm 0.59, 0.73) in C. jejuni CIP 702. Superoxide dismutase activities were very high in the Campylobacter strains, especially in C. fetus ssp. fetus [specific activity 7.8-55.7 U (mg protein)-1] compared with those in Escherichia coli (1.5 U mg-1), Propionibacterium acnes (1.6 U mg-1) and Veillonella alcalescens (0.2 U mg-1).