The human beta-globin gene contains multiple regulatory regions: identification of one promoter and two downstream enhancers.
Open Access
- 1 February 1988
- journal article
- research article
- Published by Springer Nature in The EMBO Journal
- Vol. 7 (2) , 377-384
- https://doi.org/10.1002/j.1460-2075.1988.tb02824.x
Abstract
We have introduced into murine erythroleukaemia (MEL) cells several series of deletion mutants of hybrid genes between the human beta‐globin gene and the murine H‐2Kb gene. S1 nuclease and transcriptional run‐off analysis showed that the human beta‐globin gene contains at least three globin specific transcriptional control elements. One is a promoter element and is located 160 bp upstream from the transcription initiation site; it increases the efficiency of the promoter after MEL cells are induced to differentiate. The other two elements are tissue‐specific transcriptional enhancers and are located downstream from the transcription initiation site, the first in the structural beta‐globin gene and the second in the 3′ flanking sequences.This publication has 31 references indexed in Scilit:
- Position-independent, high-level expression of the human β-globin gene in transgenic miceCell, 1987
- Activation of the human beta-globin promoter in K562 cells by DNA sequences 5' to the fetal gamma- or embryonic zeta-globin genes.Journal of Clinical Investigation, 1987
- Increased MHC H–2K gene transcription in cultured mouse embryo cells after adenovirus infectionNature, 1985
- Specific expression of a foreign β-globin gene in erythroid cells of transgenic miceNature, 1985
- DNA sequences required for regulated expression of β-globin genes in murine erythroleukemia cellsCell, 1984
- Regulated expression of the human β-globin gene family in murine erythroleukaemia cellsNature, 1983
- Three regions upstream from the cap site are required for efficient and accurate transcription of the rabbit β-globin gene in mouse 3T6 cellsCell, 1983
- The regulated expression of β-globin genes introduced into mouse erythroleukemia cellsCell, 1983
- K562—A human erythroleukemic cell lineInternational Journal of Cancer, 1979
- Human α-globin gene expression following chromosomal dependent gene transfer into mouse erythroleukemia cellsCell, 1978