Influence of cell cycle on the efficiency of transfection with purified human cytomegalovirus DNA
- 1 September 1983
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 78 (3-4) , 287-292
- https://doi.org/10.1007/bf01311323
Abstract
The efficiency of transfection of purified HCMV genome was studied in synchronized human embryo fibroblasts. The data show that HCMV DNA infection performed in S phase is more efficient than in cells infected in G2, M and G1 phases of cell cycle, respectively. These findings stress the importance of the metabolic state of cell cultures in HCMV genome transfection.Keywords
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