Improved media for growth and aerotolerance of Campylobacter fetus

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Abstract
The microaerophilic nature of C. fetus has complicated its recovery from human and animal sources. In this study, modifications of brucella agar and broth were tested for enhancement of growth and aerotolerance of 64 strains of C. fetus, representing each subspecies. Brucella agar supplemented with 0.025% each FeSO4.cntdot.7H2O, sodium metabisulfite and sodium pyruvate, supported growth of 98, 77 and 63% of the strains at 6% O2, 17% O2 and 21% O2, respectively. Unsupplemented brucella agar supported growth of 94, 48 and 20% of the strains. Brucella broth supplemented with 0.2% FeSO4.cntdot.7H2O, 0.025% sodium metabisulfite and 0.05% sodium pyruvate supported growth of 98% of the strains at 21% O2, compared to 75% with unsupplemented brucella broth. With both the supplemented agar and broth, growth responses occurred 1 to 2 days earlier than usual. Growth and aerotolerance of 3 strains of C. sputorum ssp. bubulus were not enhanced by the supplements.