Scanning Electron Microscopic Study of Skeletal Muscle

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Abstract
With scanning electron microscopy (SEM), highly organized parallel rows of pairs of openings corresponding to the level of the transverse tubular (T) system were seen on the surface of the muscle from normal mice and humans. The openings, usually round, were always found at the level of the A-I junction and varied from 500 to 1,500 Angstroms in diameter. Disappearance of the T system openings and the A-I banding pattern occurred in focal areas of both dystrophic and neurogenic atrophic fibers in the early stage. The results from scanning and transmission electron microscopy, and the preparation of specimen—of SEM are discussed.