Derivatization Procedure for Identification of Aflatoxin M1 on a Thin Layer Chromatogram
- 1 May 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 59 (3) , 722-723
- https://doi.org/10.1093/jaoac/59.3.722
Abstract
A commodity extract containing presumptive aflatoxin is placed on an origin spot of a thin layer chromatographic plate and overspotted with trifluoroacetic acid. The mixture is held in the dark 30 min at ambient temperature and then 30 min at 55°C. The plate is developed with CHCl3-acetone-2-propanol (85+10+7). The Rf values of reacted and unreacted aflatoxin M1 are compared with authentic similarly treated for identification. The lowest concentration that has been identified is 0.1 μg/kg.This publication has 0 references indexed in Scilit: