DNA sequence preference of the progesterone receptor.
- 1 January 1983
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 80 (1) , 16-20
- https://doi.org/10.1073/pnas.80.1.16
Abstract
Highly purified hen oviduct progesterone receptor A subunit was tested for binding to several chicken gene DNA. Sequence preference, detected by nitrocellulose filter adsorption of [32P]DNA fragments obtained from recombinant plasmids, revealed a marked retention of certain DNA fragments. A 10-fold preference was seen for DNa fragments flanking the 5'' end of the steroid-regulated genes ovalbumin and gene Y. No preference was seen with analogous DNA fragments from chicken .beta.-globin and .alpha.-actin genes. Restriction endonuclease mapping suggests the presence of multiple receptor interaction sites flanking the 5'' terminus of the ovalbumin gene. One of these preferential binding sites was localized between-135 -247 basepairs upstream from the start of transcription. This region contains an 18-basepair A + T-rich sequence, a likely candidate for the binding site itself, because earlier studies had shown receptor A to have marked preference for A + T-rich DNA.This publication has 18 references indexed in Scilit:
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