Catalytic Unfolding and Proteolysis of Cytochrome c Induced by Synthetic Binding Agents
- 18 September 2004
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 126 (40) , 12833-12842
- https://doi.org/10.1021/ja0317731
Abstract
A class of polyanionic copper porphyrin dimers is shown to selectively increase the susceptibility of cytochrome c to proteolysis through binding-induced disruption of tertiary and secondary structure. The free energy of the protein conformation leading to proteolytic attack is stabilized by about 2.4 kcal/mol in the bound state. The proteolytic acceleration is catalytic in nature, requiring only a fraction of an equivalent of metalloporphyrin to effect complete, rapid digestion in the presence of a protease.Keywords
This publication has 15 references indexed in Scilit:
- Binding and Catalysis: A Thermodynamic Study on a Catalytic Antibody SystemChemBioChem, 2003
- A chemical inhibitor of N-WASP reveals a new mechanism for targeting protein interactionsProceedings of the National Academy of Sciences, 2001
- Peptide and Protein Recognition by Designed MoleculesChemical Reviews, 2000
- Conformational Modulation of Electron Transfer within Electrostatic Porphyrin: Cytochrome c ComplexesThe Journal of Physical Chemistry B, 1997
- Spectrophotometric study of coproporphyrin-I complexes of copper(II) and cobalt(II)Talanta, 1995
- Relationship between local and global stabilities of proteins: site-directed mutants and chemically-modified derivatives of cytochrome cBiochemistry, 1992
- Cis-syn thymine dimers are not absolute blocks to replication by DNA polymerase I of Escherichia coli in vitroBiochemistry, 1990
- Correlation between sites of limited proteolysis and segmental mobility in thermolysinBiochemistry, 1986
- Total synthesis of (.+-.)-4-amino-4-deoxychorismic acid: a key intermediate in the biosynthesis of p-aminobenzoic acid and L-p-aminophenylalanineJournal of the American Chemical Society, 1985
- Aggregation of nickel(II), copper(II), and zinc(II) derivatives of water-soluble porphyrinsInorganic Chemistry, 1973