A Stable, High-Contrast Mordant for Hematoxylin Staining
- 1 January 1936
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 11 (4) , 149-151
- https://doi.org/10.3109/10520293609110516
Abstract
Small, quantities of sulfuric acid will stabilize iron mordants, used in hematoxylin staining, by preserving these solutions against oxidation. The presence of acetic acid in the mordant improves the specificity of the stain. A stable, high-contrast mordant is obtained when both acids are combined with ferric-ammonium sulfate. This mordant, used in combination with fresh alkaline solutions of hematoxylin, has been found especially effective in the staining of certain nuclear and cytoplasmic components of plant cells.Keywords
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