Recognition of base pairs by polar peptides in double stranded DNA
- 1 January 1982
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (5) , 1707-1720
- https://doi.org/10.1093/nar/10.5.1707
Abstract
The resonance Raman spectrum of native DNA has been obtained using excitation at 257 nm. In a first part, the spectral lines are assigned to the different nucleotide bases which provide the resonance effect. In a second part, the interactions of DNA with basic peptides (Arginine Methylester, Lysine Methylester, Arginyl-Arginine) are investigated using excitation at 300 nm and 257 nm, which give complementary information about the DNA. Both Arginine Methylester and Arginyl-arginine recognize the A-T base pairs, the first one in the large groove, the second one in the narrow groove of DNA. The DNA-Lysine Methylester interaction is very likely not specific but can take place in the large groove of DNA.Keywords
This publication has 23 references indexed in Scilit:
- Nucleosome structure: sites of interaction of proteins in the DNA grooves as determined by Raman scatteringBiochemistry, 1979
- Laser Raman identification of an interaction site on DNA for arginine containing histones in chromatinBiochemical and Biophysical Research Communications, 1976
- Stereospecific binding of diastereomeric peptides to salmon sperm DNABiochemistry, 1976
- Nucleosome formation abolishes base-specific binding of histonesNature, 1975
- Chromatin neutron and X-ray diffraction studies and high resolution melting of DNA-histone complexesBiochimie, 1974
- Associations DNA-ribonucléase: II — Etude conformationnelleBiochimie, 1974
- Conformational dependence of the Raman scattering intensities from polynucleotides. III. Order‐disorder changes in helical structuresBiopolymers, 1971
- Deoxyribonucleic acid-polylysine complexes. Structure and nucleotide specificityBiochemistry, 1969
- Binding of small cations to deoxyribonucleic acid. Nucleotide specificityBiochemistry, 1969
- Model nucleoprotein complexes: Studies on the interaction of cationic homopolypeptides with DNAJournal of Molecular Biology, 1967