An ethanol inducible gene switch for plants used to manipulate carbon metabolism
- 1 February 1998
- journal article
- Published by Springer Nature in Nature Biotechnology
- Vol. 16 (2) , 177-180
- https://doi.org/10.1038/nbt0298-177
Abstract
Many transgenic plant studies use constitutive promoters to express transgenes. For certain genes, deleterious effects arise from constant expression in all tissues throughout development. We describe a chemically inducible plant gene expression system, with negligible background activity, that obviates this problem. We demonstrate its potential by showing inducible manipulation of carbon metabolism in transgenic plants. Upon rapid induction of yeast cytosolic invertase, a marked phenotype appears in developing leaves that is absent from leaves that developed before induction or after it has ceased.Keywords
This publication has 26 references indexed in Scilit:
- A glucocorticoid‐mediated transcriptional induction system in transgenic plantsThe Plant Journal, 1997
- Chemically inducible promoters in transgenic plantsCurrent Opinion in Biotechnology, 1996
- Regulation of Metabolism in Transgenic PlantsAnnual Review of Plant Biology, 1995
- Impaired photoassimilate partitioning caused by phloem-specific removal of pyrophosphate can be complemented by a phloem-specific cytosolic yeast-derived invertase in transgenic plants.Plant Cell, 1995
- Correct intron splicing generates a new type of a putative zinc‐binding domain in a transcriptional activator of Aspergillus nidulansFEBS Letters, 1991
- Fos and Jun oncogenes transactivate chimeric or native promoters containing AP1/GCN4 binding sites in plant cells.Plant Cell, 1990
- Control of metabolism: What do we have to measure?Trends in Biochemical Sciences, 1987
- Regulation Of Gene Expression In Higher PlantsAnnual Review of Plant Biology, 1987
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962