CYTOPLASMIC NUCLEOPROTEIN OF LIVING NERVE CELLS GROWN IN VITRO

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Abstract
Explants of chick embryo brain, spinal cord and spinal ganglia were grown in tissue culture. Ultraviolet photomicrographs were made of living neurons at 265 m[mu]. Neurons are characterized by a high density and a homogenous distribution of absorbing material in the cytoplasm in UV photomicrographs. Only after fixation with 10% formalin or ethanol do Nissl bodies or cytoplasmic aggregates appear in UV photomicrographs. These clumps stain with basic dyes at low pH. Microspectrophotometry of the cytoplasm of living neurons yields an absorption spectrum like that of nucleoprotein in solution with an absorption maximum close to 265 m[mu] and a lesser peak at 280 m[mu]. There is no alteration in shape of this absorption spectrum after fixation.

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