Oestrogen Receptors in Macrophages

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Abstract
The presence of receptors for oestradiol-17.beta. and 5.alpha.-dihydrotestosterone (5.alpha.-DHT) in the human monocytic leukaemia cell line J111 and rat peritoneal macrophages were investigated using whole-cell assays. For both cell types, high-affinity binding species for oestrogen were detected, whereas no indication of sepcific binding was observed for 5.alpha.-DHT. Analysis of the data according to Scatchard showed curved lines, indicating the presence of two different oestrogen-binding species. The dissociation constant (Kd) values for the receptors of the rat peritoneal macrophages were calculated to be 1.4 .times. 10-10 M and 3.3 .times. 10-9 M, while for the J111 cells, the Kd values were 8.7 .times. 10-11 M and 2.5 .times. 10-9 M. Sucrose-gradient ultracentrifugation identified one oestrogen-binding species of 7.1S. The receptors had a relatively high affinity for dietylstilboestrol (DES) but did not bind to a monoclonal antibody specific for the classical oestrogen receptor, suggesting that oestrogen receptors in macrophages could be of a different type.