TUMOR-PROMOTING PHORBOL DIESTER-INDUCED SPECIFIC CHANGES IN CELL-SURFACE GLYCOPROTEIN PROFILE OF K562-HUMAN LEUKEMIC-CELLS
- 1 January 1981
- journal article
- research article
- Vol. 41 (11) , 4621-4628
Abstract
The tumor-promoting phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), induced specific changes in the cell surface glycoprotein profile of K562 human leukemic cells. TPA-treated K562 cells were examined by cell surface labeling followed by immunoprecipitation with anti-glycophorin antiserum or anti-K562 antiserum. TPA-treated K562 cells lost glycophorin and glycoprotein with a MW of 105,000, which are specific to later stages of erythroid cell differentiation, and retained glycoprotein with a MW of 95,000, which is specific to early stages of erythroid cell differentiation. 4.alpha.-Phorbol-12,13-didecanoate, which is inactive as a tumor promoter, induced no significant changes in cell surface glycoproteins of K562 cells. TPA induced morphological changes of K562 cells as their growth was inhibited, whereas 4.alpha.-phorbol-12,13-didecanoate had no effect on these 2 characteristics. The tumor promoter induced specific changes in the cell surface glycoprotein profile of K562 human leukemic cells, which appeared to be induced into a retrodifferentiated stage.This publication has 27 references indexed in Scilit:
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