A NEW HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC PROCEDURE FOR THE SEPARATION AND QUANTITATION OF VARIOUS HEMOGLOBIN-VARIANTS IN ADULTS AND NEWBORN BABIES

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  • 1 January 1983
    • journal article
    • research article
    • Vol. 102  (2) , 174-186
Abstract
A new cation-exchange HPLC [high performance liquid chromatography] method is described for the separation and quantitation of abnormal human Hb. The method makes use of Synchropak CM 300, a silica support with carboxylic acid residues, and Bis-Tris-KCN-Na-acetate developers and allows a completion of the chromatogram in 50-90 min depending on the sample to be analyzed. Specific chromatographic profiles were obtained for several .beta., .alpha. and .delta. chain variants. The method is useful not only for the quantitation of Hb F and Hb A2 (except in the presence of Hb E) but also provides easy differentiation between simple heterozygotes for a specific abnormality and persons with the same abnormality together with an additional .beta.-thalassemia heterozygosity. Probably the most important application of the new procedure is in the quantitation of Hb A and the .beta. chain variants S, C, O-Arab, and E in cord blood samples, thus facilitating the diagnosis of conditions such as AE, EE, AS, SS, S-.beta.+thal, AC, CC, C-.beta.+thal, SC and SO in newborn babies.

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