Cupredoxines of obligate methylotroph

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Abstract
Cells of type I obligate methylotroph Methylobacillus flagellatum KT, which can use only methanol or methylamine as carbon and energy sources, possess two types of blue copper proteins: amicyanin and azurin. During exponential phase of growth amicyanin was the only blue copper‐containing protein in the cells from methylamine medium. Azurin‐type proteins appeared in these cells when they reached the stationary growth phase. In contrast, amicyamin could not be detected in methylamine‐grown cells when they are from stationary growth phase. Cells from methanol‐containing medium possess only azurin, amicyanin could not be detected in all growth phases. Kinetic studies with methylamine dehydrogenase and amicyanin purified from M. flagellatum KT cells show that amicyanin may serve as a primary electron acceptor from methylamine and methylamine dehydrogenase in this type I of obligate methylotroph.