Mixed Lymphocyte Reactivity and Cell-Mediated Lympholysis to D-End Differences of the Murine Major Histocompatibility Complex.

Publisher Website
Google Scholar
Abstract
In vitro mixed lymphocyte culture (MLC) responses and the in vitro induction of cell-mediated lympholysis (CML) were studied in congenic strain combinations in which the responding and stimulating strains differed at the entire major histocompatibility complex (MHC) or only at the D end of the MHC. The relative strengths of stimulation by D end only differences or by whole MHC differences were examined by stimulating identical responding populations with titrated numbers of stimulating cells that differed from the responder at the D end only or over the entire MHC. Isolated D-end differences were sufficient to generate significant MLC and CML responses in each combination tested. Several D end only differences (the responses of B10.A to B10.A [2R], of B10.A [2R] to B10.A, of B10.D2 to B10.HTG and of B10.HTG to B10.D2) were severalfold less efficient in stimulating MLC and CML responses than were control stimulating cells differing over the whole MHC. When the mutant D-end allele da was present on the stimulating cell (as seen in the responses of B10.D2 to B10.D2 [M504] and of B10 to B10.D2 [R106], stimulation by an isolated D-end differences was comparable to stimulation by broader MHC differences. These findings are discussed in terms of the possible functional complexity of the D region.