Photoactivated aflatoxins are mutagens to Salmonella typhimurium and bind covalently to DNA in vitro

31 December 1981

journal article
research article
Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research

Vol. 3 (4) , 423-429
https://doi.org/10.1093/carcin/3.4.423

Abstract

The mutagenesis of Salmonella typhimurium TA100 and covalent binding in vitro of photoactivated aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1) and aflatoxin G2 (AFG2) were investigated. Covalent binding levels of 1140, 225, 330 and 8 pmol aflatoxin/.mu.mol nucleotide phosphate were obtained for AFB1, AFG1, AFB2 and AFG2, respectively, at 50 .mu.M mycotoxin after 2 h of irradiation. Mutant frequencies to histidine prototrophy were 97, 19, 49 and 0 .times. 10-6 for AFB1, AFG1, AFB2 and AFG2, respectively, after 2 irradiation at 100 .mu.M mycotoxin in the surviving fraction of the mutagenized cultures. Toxicity to Salmonella was 0.59, 0.03, 0.31 and 0 lethal hits under the conditions specified for mutagenesis for AFB1, AFG1, AFB2 and AFG2, respectively.

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