Restored invasion of mouse MO4 cells into chick heartin vitro through mutual conditioning at reduced temperature

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Abstract
Invasion of malignant mouse MO4 cells into embryonic chick heart fragments in confronting organ cultures was arrested for 7 days when the temperature of incubation was lowered to 28°C. Afterwards invasion resumed and progression between days 10 and 17 at 28°C was comparable to that between days 0 and 7 at 37°C. This pattern of progression of MO4 cell invasion at 28°C was unaltered when either MO4 cells or heart fragments or both were preincubated separately at 28°C for 14 days before confrontation with each other. Invasion at 28°C resumed only when MO4 cells and heart tissue had been in immediate contact for at least 7 days. Metabolic labelling with [3H]fucose showed a correlation in time between transient suppression of invasion and transient inhibition of incorporation of fucosylation-precursor molecules into glycoproteins by MO4 cells. The latter activity was far less temperature-sensitive in heart cells. Our observations suggest that metabolic cooperation between invading MO4 cells and heart tissue is essential for progression of invasionin vitro.