On Iodometric and Polarimetric Determination of Penicillin.

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Abstract
A modified iodometric method of improved accuracy for the detn. of penicillin is described wherein the standard deviation for a single sample is about [plus or minus] 1.5%. The pH of the iodine reaction mixture is 4.5; both blank and alkali-inactivated penicillin, are treated with I2 for 20 min. Penicillin consumes 8 equivalents of I2 per mole. Polarimetric detn. is satisfactory when active penicillin is used and no other optically active compounds are present. The quantitative inactivation of penicillin may be detd. from reduction of rotation only when the reaction is run at a known constant pH. Under certain conditions it is possible to use the iodometric method for the detn. of the penicillin content of broths.

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