Radioimmunoassay of creatine kinase isoenzymes in human serum: isoenzyme BB.
- 1 March 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 24 (3) , 422-428
- https://doi.org/10.1093/clinchem/24.3.422
Abstract
We describe a sensitive, specific radioimmunoassay for the BB isoenzyme of creatine kinase (CK-BB) in serum. A sequential saturation assay was used to achieve sufficient sensitivity to detect the isoenzyme in 100-microliter serum samples of all healthy persons and patients tested. Bound and free antigen were separated by a second antibody system. Large excesses of purified isoenzyme MM did not react in the assay. Cross reactivity of two preparations of CK-MB was only 1 to 7+. The 95th percentile of serum CK-BB in 208 healthy adults was 6.2 microgram/liter. Within-assay and between-assay precision ranged from 5.5 to 11.9% and 9.7 to 13.6%, respectively.This publication has 4 references indexed in Scilit:
- Radioimmunoassay of creatine kinase-B isoenzyme in human sera: results in patients with acute myocardial infarction.Proceedings of the National Academy of Sciences, 1977
- Brain-Type Creatine Kinase IsoenzymeArchives of Neurology, 1977
- Detection of creatine kinase BB isoenzyme in sera of patients undergoing aortocoronary bypass surgeryClinica Chimica Acta; International Journal of Clinical Chemistry, 1977
- Serum creatine kinase isoenzyme pattern in nervous system atrophies and neuromuscular disordersClinica Chimica Acta; International Journal of Clinical Chemistry, 1976