Human epidermal growth factor: isolation and chemical and biological properties.
Open Access
- 1 April 1975
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (4) , 1317-1321
- https://doi.org/10.1073/pnas.72.4.1317
Abstract
A polypeptide hormone has been isolated from human urine, human epidermal growth factor. It was assayed by its ability to compete with 125I-labeled mouse-derived epidermal growth factor in binding to human foreskin fibroblasts. The biological effects of the human polypeptide are similar to those previously described for the mouse hormone. These include the stimulation of the growth in vitro of human foreskin fibroblasts and corneal epithelial cells in organ culture, and the in vivo induction of precocious eyelid opening in the newborn mouse. The amino acid compositions of the two polypeptides differ, although certain similarities are present. The estimated molecular weight of the human polypeptide, 5300-5500, is slightly lower than that of the mouse hormone. Both polypeptides apparently compete for the same site on the cell membrane; and antibodies to the mouse polypeptide crossreact to some extent with the human hormone.Keywords
This publication has 11 references indexed in Scilit:
- Epidermal growth factor. Location of disulfide bonds.1973
- Epidermal Growth Factor: Receptors in Human Fibroblasts and Modulation of Action by Cholera ToxinProceedings of the National Academy of Sciences, 1973
- Pituitary Extracts and Steroid Hormones in the Control of 3T3 Cell GrowthProceedings of the National Academy of Sciences, 1973
- Proliferation of corneal epithelium induced by epidermal growth factorExperimental Eye Research, 1973
- Isolation and characterization of renin-like enzymes from mouse submaxillary glandsBiochemistry, 1972
- A potential sourse of electrophoretic artifacts in polyacrylamide gelsBiochimica et Biophysica Acta (BBA) - Protein Structure, 1967
- Isolation of a Mouse Submaxillary Gland Protein Accelerating Incisor Eruption and Eyelid Opening in the New-born AnimalJournal of Biological Chemistry, 1962
- Specific Volumes of Proteins and the Relationship to their Amino Acid ContentsScience, 1952
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951
- The spectrophotometric determination of tyrosine and tryptophan in proteinsBiochemical Journal, 1946