Rate of Respiration of Tissues in Contact with Oxygen.

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Abstract
A method is descr. for the detn. of the respiratory rates of tissues in contact with O2 by supporting them in the gas phase on fiber-glass mats in a modified wide-mouthed Warburg flask. (E. Machlett and Co., New York City.) The tissues maintained a satisfactory physiologic state as evidenced by slow decline in respiratory rate and negligible changes in pH. For 8 rat tissues studied the respiratory rates on nuts were considerably higher than those in Krebs-Ringer-phosphate; and higher than those in Krebs fortified Medium III in the case of muscle, brain, kidney, and heart, lower only for spleen, and not significantly different for liver, intestine and testis. Application of the respiratory rates to summated tissue calculations yielded the following values relative to the basal metabolic rate: on mats, 101.8%; in Krebs-Ringer-phosphate, 78.7%; and in Krebs Medium III, 90.7%. The utility of the technique using mats as a means of avoiding variables due to different liquid media is discussed.

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