Characterization of Pyridoxine Auxotrophs ofEscherichia coli: Serine and PdxF Mutants

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Abstract
At least six phenotypically distinct classes of mutants of Escherichia coli which require serine or pyridoxine or both can be isolated. Three of the six classes lack 3-phosphoserine-2-oxoglutarate aminotransferase. One of these classes contains WG5, a mutant previously characterized as containing the pdxF5 allele. The aminotransferase isolated from this mutant has been compared to that isolated from wild-type E. coli and found to have apparently normal affinity for pyridoxal 5′-phosphate, but reduced affinity for pyridoxamine 5′-phosphate.

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