The lac repressor and its N‐terminal headpiece can bind a mini‐operator containing a hairpin loop made of a hexaethylene glycol chain
- 19 August 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 288 (1-2) , 101-104
- https://doi.org/10.1016/0014-5793(91)81012-w
Abstract
The binding of the lac repressor and the lac repressor N-terminal headpiece to a mini-operator with a hairpin loop made of a hexaethylene glycol chain was investigated using circular dichroism spectroscopy. The Iac repressor's headpiece binds to the modified mini-operator with the same affinity as to a mini-operator of the same sequence without the hexaethylene glycol loop. The conformational effect due to the binding is not affected by the presence of the hexaethylene loop. It is also shown that the entire lac repressor binds to this modified mini-operator inducing a conformational change.Keywords
This publication has 33 references indexed in Scilit:
- Assignment of the 1H‐NMR spectrum of a lac repressor headpiece‐operator complex in H2O and identification of NOEsEuropean Journal of Biochemistry, 1990
- Hairpin structures in DNA containing arabinofuranosylcytosine. A combination of nuclear magnetic resonance and molecular dynamicsBiochemistry, 1990
- Hairpin structures in synthetic oligodeoxynucleotides: sequence effects on the duplex-to-hairpin transitionBiochimie, 1989
- Proton NMR study of a complex between the lac repressor headpiece and a 22 base pair symmetric lac operatorBiochemistry, 1989
- Oligodeoxynucleotide folding in solution: loop size and stability of B-hairpinsBiochemistry, 1988
- X-ray structure of a DNA hairpin moleculeNature, 1988
- NUCLEAR MAGNETIC RESONANCE AND DISTANCE GEOMETRY STUDIES OF DNA STRUCTURES IN SOLUTIONAnnual Review of Biophysics, 1987
- Complex of lac repressor headpiece with a 14 base-pair lac operator fragment studied by two-dimensional nuclear magnetic resonanceJournal of Molecular Biology, 1987
- NMR Study of the Interaction between the lac Repressor and the lac OperatorEuropean Journal of Biochemistry, 1983
- DNA-binding site of lac repressor probed by dimethylsulfate methylation of lac operatorJournal of Molecular Biology, 1979