DIFFERENTIAL REGULATION OF SPLEEN CELL-MEDIATED EOSINOPHIL AND NEUTROPHIL-MACROPHAGE PRODUCTION

  • 1 January 1980
    • journal article
    • research article
    • Vol. 55  (3) , 489-493
Abstract
Non-adherent spleen cells of mice infected with Trichinella spiralis released growth stimulatory factors (GSF) in vitro when challenged with excretory/secretory products of muscle stage larvae. The assay of GSF was based on proliferation of normal, non-adherent syngeneic marrow cells in liquid tube cultures. Media conditioned for 1 day by challenged spleen cells stimulated eosinophil production but failed to stimulate production of other cell types. Media conditioned for 5 days supported eosinophil, neutrophil and macrophage production. Kinetics of cell production were different. Eosinophil production started within 1 day, reached a peak at day 2 and was down to control levels by day 4. Neutrophil/macrophage production began between 2 and 4 days and reached a peak at 6-8 days. The short duration of eosinophil production was evidently due to depletion of growth-factor-responsive cells.