An Assay for the Mitogenic Activity of Phytohemagglutinin Preparations
Open Access
- 1 November 1965
- journal article
- research article
- Published by American Society of Hematology in Blood
- Vol. 26 (5) , 569-578
- https://doi.org/10.1182/blood.v26.5.569.569
Abstract
The addition of increasing levels of phytohemagglutinin to amethopterin-treated cultures of human lymphocytes results in the induction of DNA synthesis which can be measured by the incorporation of exogenous radioactive thymidine. The graded nature of this response permits the assay of mitogenic activity of such preparations as well as the assessment of antimitogenic factors in sera.This publication has 6 references indexed in Scilit:
- CELL TRANSFORMATIONS AND MITOSES PRODUCED IN VITRO BY TUBERCULIN PURIFIED PROTEIN DERIVATIVE IN HUMAN BLOOD CELLSPublished by Elsevier ,1963
- The Effect of Phytohemagglutinin on Leukocyte Cultures as Measured by P32 Incorporation in the DNA, RNA, and Acid Soluble FractionsBlood, 1962
- The Kinetics of Cell Proliferation in Cultures of Human Peripheral BloodBlood, 1962
- MOLECULAR EVENTS IN REPRODUCTION OF ANIMAL CELLS .1. EFFECT OF PUROMYCIN ON DUPLICATION OF DNA1962
- STUDIES ON UNBALANCED GROWTH IN TISSUE CULTURE .1. INDUCTION AND CONSEQUENCES OF THYMIDINE DEFICIENCY1960
- PHYTOHEMAGGLUTININ - AN INITIATOR OF MITOSIS IN CULTURES OF NORMAL HUMAN LEUKOCYTES1960