Differentiation of Measles Virus Strains and a Strain of Canine Distemper Virus by Monoclonal Antibodies

Publisher Website
Google Scholar
Abstract
Monoclonal antibodies raised against the Halle strain of measles virus were tested for their strain specificity using other measles virus isolates (Edmonston, Leningrad, Lec and 2 fresh isolates , JT and Fasquelle). Monoclonal antibodies to L, HA and NP polypeptides, with 1 exception, reacted with all the measles virus strains tested; antibody from hybrid lines 25 failed to react with the NP polypeptide of JT virus by immunofluorescence or radioimmunoprecipitation. Three other monoclonal antibodies, 2 reacting with NP and 1 with HA, although giving a positive immunofluorescence reaction, did not immunoprecipitate labeled antigen from JT virus-infected [African green monkey kidney BGM] cells. The immunological relationship of canine distemper and measles viruses was also investigated with monoclonal antibodies. Two of 3 anti-NP and 3 of 4 anti-HA monoclonal antibodies reacted with canine distemper virus in an immunofluorescence reaction but only 1 (anti-NP) reacted in a radioimmunoprecipitation test.