Determination of Acetylcholinesterase and Pseudocholinesterase in Gastrointestinal Biopsy Tissue
- 1 January 1982
- journal article
- research article
- Published by Walter de Gruyter GmbH in cclm
- Vol. 20 (2) , 69-74
- https://doi.org/10.1515/cclm.1982.20.2.69
Abstract
An optimized micromethod for the determination of soluble acetylcholinesterase and pseudocholinesterase in [human] gastrointestinal biopsy tissue is introduced. Enzyme activities were obtained from measurements of total activity and activity in the presence of an inhibitor of pseudocholinesterase. Optimal conditions of measurement were established for enzymes in serum or erythrocytes. In intestinal tissue, measurements were precise and reproducible. Interference by blood contained in the biopsy specimens was neglegible. Drugs used for premedication had no significant influence. Results from normal stomach, duodenum and colon also are presented. The use of cholinesterase determinations in physiological studies and the diagnosis of Hirschsprung disease are discussed.This publication has 11 references indexed in Scilit:
- Histochemical diagnosis of Hirschsprung's disease and a comparison of the histochemical and biochemical activity of acetylcholinesterase in rectal mucosal biopsies.Journal of Clinical Pathology, 1980
- Acetylcholinesterase activity and its molecular forms in rectal tissue in the diagnosis of hirsprung's diseaseClinica Chimica Acta; International Journal of Clinical Chemistry, 1979
- DIAGNOSTIC VALUE OF RECTAL MUCOSAL ACETYLCHOLINESTERASE LEVELS IN HIRSCHSPRUNG'S DISEASEThe Lancet, 1979
- An improved method for the determination of acetylcholinesterase activity in rectal biopsy tissue from patients with hirschsprung's diseaseClinica Chimica Acta; International Journal of Clinical Chemistry, 1977
- Autonomic nerves in rectum and colon in Hirschsprung's disease. A cholinesterase and catecholamine histochemical study.Archives of Disease in Childhood, 1969
- Eine kinetische Methode zur Bestimmung der Aktivität der Pseudocholinesterase (Acylcholin-acylhydrolase 3.1.1.8.)Klinische Wochenschrift, 1967
- Rasche und einfache Ultramikromethode zur Bestimmung der SerumcholinesteraseDeutsche Medizinische Wochenschrift (1946), 1966
- A Titrimetric Method for the Determination of Plasma and Red Blood Cell Cholinesterase Activity Using Thiocholine Esters as SubstratesScandinavian Journal of Clinical and Laboratory Investigation, 1955
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951
- THE EFFECT OF INHIBITION OF SPECIFIC AND NON-SPECIFIC CHOLINESTERASE ON THE MOTILITY OF THE ISOLATED ILEUM1950